The effect of fucoxanthin on the development of CCl<sub>4</sub>-induced liver fibrosis

Background . According to current concepts regarding hepatic fibrosis, myofibroblast differentiation from stellate cells, regulated by transforming growth factor-β (TGF-β), is a key step in its pathogenesis. Hence, inhibition of TGF-β-dependent activation cells has been suggested as promising strategy for preventing the disease development. Aim To explore whether administration fucoxanthin at dose 30 mg/kg efficient suppressing fibrosis. Materials and Methods The experiments were carried out on outbred ICR/CD1 mice which have divided into three groups: intact animals, animals with untreated fibrosis induced intraperitoneal injections CCl 4 (2 μl/g, 6 weeks, twice per week), received os (30 daily 5 weeks) after inducing described above. Histological examination was performed Sirius Red staining using METAVIR activity score. Immunohistochemical analysis quantitation α-SMA-positive myofibroblasts, CD45-positive leukocytes, TIMP-1-positive regions. Further, we quantified TGF-β liver homogenate well interleukin-1β (IL-1β) tumor necrosis factor-α (TNF-α) serum means enzyme-linked immunosorbent assay. An assessment function conducted measuring alanine aminotransferase, aspartate albumin levels. Results Fucoxanthin decreased number myofibroblasts volume connective tissue regions, level homogenate, altogether indicative ameliorated In accord, treatment reduced IL-1β, TNF-α, aminotransferase increased albumin. Conclusion Treatment an antifibrotic effect diminishes